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mpc1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mpc1 antibody
    (A) Western blot image of <t>MPC1</t> at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.
    Mpc1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpc1+antibody/MPC1+Rabbit+mAb/bio_rxiv__64898__2026__02__03__703651-92-15-17
    Average 95 stars, based on 102 article reviews
    mpc1 antibody - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions"

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    Journal: bioRxiv

    doi: 10.64898/2026.02.03.703651

    (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.
    Figure Legend Snippet: (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.

    Techniques Used: Western Blot, Control, Quantitative Proteomics

    (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.
    Figure Legend Snippet: (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.

    Techniques Used: Western Blot

    (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.
    Figure Legend Snippet: (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.

    Techniques Used: Lysis, Membrane, Isolation, Western Blot, Control

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    Article Snippet: Myosin VIIa 1:500 Rabbit Polyclonal Proteus Biosciences 25-6790 Pou4f3 1:200 Mouse monoclonal Santa Cruz sc-81980 3 HA-Tag 1:400 Rabbit monoclonal Cell Signaling Technology 3724 MPC1 1:200 Rabbit monoclonal Cell Signaling Technology 14462 MPC2 1:200 Rabbit monoclonal Cell Signaling Technology 46141 ACO2 1:200 Rabbit monoclonal Cell Signaling Technology 6571 Dlst 1:200 Rabbit monoclonal Cell Signaling Technology 11954 Sdha 1:200 Rabbit monoclonal Cell Signaling Technology 11998 Beta-Actin 1:1000 Rabbit monoclonal Cell Signaling Technology 4970 DAPI 1:2000 Fluorescent labeled nucleus Roche 28718-90- 3 4 Alexa Fluor 568 goat anti-mouse lgG1 1:500 Secondary antibodies Life Technologies A21124 488-affinipire goat anti-rabbit IgG 1:500 Secondary antibodies Jackson 111-545- 003 647-affinipire goat anti-mouse IgG 1:500 Secondary antibodies Jackson 111-605- 003 594-affinipire goat anti-mouse IgG 1:500 Secondary antibodies Jackson 115-585- 003

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    Membrane:

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions
    Article Snippet: .. The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight. .. The secondary goat anti-rabbit IgG antibody for MPC1 and MPC2 was diluted 1:10,000 (Millipore Sigma Ref AP132P) and the secondary goat anti-mouse IgG antibody for the other proteins were diluted 1:2000 (Millipore Sigma Ref AP127P).



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    (A) Western blot image of <t>MPC1</t> at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.
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    Image Search Results


    (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Western blot image of MPC1 at 12 kD in the soluble fraction from 37 to 66°C of whole cells treated with vehicle control and UK5099. (B) The western blot was quantified using Image Lab.Relative intensities were used to quantify protein abundance normalized to 37°C. Curves fitted to the Boltzmann Sigmoid equation and demonstrate no statistically significant difference between the melting temperature of MPC1 in the control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.91). (C) Western blot image of MPC at 12 kD in the soluble fraction of whole cell lysate treated with vehicle control and UK5099. (D) Quantified protein abundance in the soluble fraction fitted to the Boltzmann Sigmoid equation demonstrate no significant difference in the melting temperatures between control and UK5099-treated conditions (n = 1, N = 3, extra sum of squares F test, p = 0.82). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Western Blot, Control, Quantitative Proteomics

    (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Western blot image of MPC1 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (B) The western blot was quantified as described above. The DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, paired t-test assuming unequal variance, * = p < 0.05, ** = p < 0.01). (C) Western blot image of MPC2 in the soluble fraction between the temperatures of 37 and 66°C in DMSO and UK5099-treated conditions. (D) The western blots were quantified as above and the DMSO samples were fitted to a Boltzmann Sigmoid curve and compared to the UK5099 samples (n = 1-2, N = 3, extra sum of squares F-test, p < 0.05). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Western Blot

    (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.

    Journal: bioRxiv

    Article Title: Cellular thermal shift assay of subcellular isolates for evaluating drug-membrane target interactions

    doi: 10.64898/2026.02.03.703651

    Figure Lengend Snippet: (A) Schematic representing hypothesis that cellular lysis disrupts mitochondrial membrane integrity whereas with use of mitochondrial isolation it is preserved. (B) Western blot image of MPC1 in the soluble fraction between the temperatures 37 and 66°C in control versus UK5099 treated samples. (C) The samples were fitted to the Boltzmann Sigmoid curve, and no significant difference was observed in the MPC1 melting temperature between control and UK5099-treated conditions (n = 1-2, N = 3, extra sum of square F test, p = 0.7611). Blot images are representative of means obtained from independent replicates.

    Article Snippet: The membrane was blocked in 2% BSA for two days before being probed with 1:500 MPC1 antibody (Cell Signaling Ref 14462) [ ], 1:2000 MPC2 antibody (Cell Signaling Ref 46141) [ ], 1:2000 Porin VDAC antibody (Calbio Ref 529534), or 1:2000 ß-actin antibody (Cell Signaling Ref 3700) overnight.

    Techniques: Lysis, Membrane, Isolation, Western Blot, Control

    (A) Generation of BAT-specific Got1 knockout mice. Exon 2 of the mouse GOT1 gene was targeted for deletion by the LoxP/Cre system. (B) qPCR analysis for detection of Got1 transcripts in Got1 fl/fl and Got1 BATKO male mice exposed to 4°C for 7 days (n=6-7/group). (C) WB analysis for GOT1 in Got1 fl/fl and Got1 BATKO mice housed at 23°C or exposed to 4°C for 7 days. (D) Body temperature of Got1 fl/fl and Got1 BATKO female mice during cold exposure (n=7-8/group). Core body temperature was measured using a rectal thermometer at indicated times. (E) WB and qPCR of UCP1 in BAT from the same mice described in D. (F) Glucose tolerance test of cold exposed- Got1 fl/fl and Got1 BATKO female mice (n=7-10/group). (G) Measurement of [ 3 H]-2DG uptake by cold-activated BAT. (H) The NAD + /NADH ratio in BAT homogenates. (I-M) Measurement of body weight, energy expenditure, RER, food intake, and locomotor activity in Got1 fl/fl and Got1 BATKO male mice (n=6/group). Mice were placed in indirect calorimetric chambers and monitored during β-adrenergic stimulation of BAT with a β 3 AR agonist CL316243 (1mg/kg BW, daily). (N) Oxidation of 14 C-labeled palmitate or pyruvate in BAT homogenates. BAT was extracted from mice exposed to 4°C for 7 days. (O) qPCR analysis of genes involved in glucose uptake, glycolysis, and FA oxidation in cold-activated BAT (n=6/group). (P) Uptake of 14 C-labeled pyruvate by the mitochondria isolated from cold-activated BAT. (Q) Reduced phosphorylation of the PDH E1α subunit at Ser232 with a concurrent increase in PDH activity in Got1-deficient BAT. (R) Measurement of pyruvate-dependent mitochondrial respiration in BAT explants extracted from mice exposed to 4°C for 7 days. All data are presented as the Mean ± SEM. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001, # p <0.0001.

    Journal: bioRxiv

    Article Title: Cold-inducible GOT1 activates the malate-aspartate shuttle in brown adipose tissue to support fuel preference for fatty acids

    doi: 10.1101/2024.11.18.623867

    Figure Lengend Snippet: (A) Generation of BAT-specific Got1 knockout mice. Exon 2 of the mouse GOT1 gene was targeted for deletion by the LoxP/Cre system. (B) qPCR analysis for detection of Got1 transcripts in Got1 fl/fl and Got1 BATKO male mice exposed to 4°C for 7 days (n=6-7/group). (C) WB analysis for GOT1 in Got1 fl/fl and Got1 BATKO mice housed at 23°C or exposed to 4°C for 7 days. (D) Body temperature of Got1 fl/fl and Got1 BATKO female mice during cold exposure (n=7-8/group). Core body temperature was measured using a rectal thermometer at indicated times. (E) WB and qPCR of UCP1 in BAT from the same mice described in D. (F) Glucose tolerance test of cold exposed- Got1 fl/fl and Got1 BATKO female mice (n=7-10/group). (G) Measurement of [ 3 H]-2DG uptake by cold-activated BAT. (H) The NAD + /NADH ratio in BAT homogenates. (I-M) Measurement of body weight, energy expenditure, RER, food intake, and locomotor activity in Got1 fl/fl and Got1 BATKO male mice (n=6/group). Mice were placed in indirect calorimetric chambers and monitored during β-adrenergic stimulation of BAT with a β 3 AR agonist CL316243 (1mg/kg BW, daily). (N) Oxidation of 14 C-labeled palmitate or pyruvate in BAT homogenates. BAT was extracted from mice exposed to 4°C for 7 days. (O) qPCR analysis of genes involved in glucose uptake, glycolysis, and FA oxidation in cold-activated BAT (n=6/group). (P) Uptake of 14 C-labeled pyruvate by the mitochondria isolated from cold-activated BAT. (Q) Reduced phosphorylation of the PDH E1α subunit at Ser232 with a concurrent increase in PDH activity in Got1-deficient BAT. (R) Measurement of pyruvate-dependent mitochondrial respiration in BAT explants extracted from mice exposed to 4°C for 7 days. All data are presented as the Mean ± SEM. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001, # p <0.0001.

    Article Snippet: PDH E1α antibody (#3205), MPC1 antibody (#14462), MPC2 antibody (#46141), GLUT1 antibody (#12939), phospho-AMPKα (T172) antibody (#2531L), AMPKα antibody (#2532), phospho-ACC (S79) antibody (#3661), and ACC antibody (#3662) were purchased from Cell Signaling.

    Techniques: Knock-Out, Activity Assay, Labeling, Isolation